What You Need to Know
The technique cools a 70–80 % w/w sucrose solution to –80 °C – –196 °C, suppressing ice nucleation with cryoprotectants (e.g., glycerol, sorbitol) to allow controlled nucleation and slow crystal growth. The low temperature and controlled cooling rate yield large, glassy sucrose crystals while preventing vitrification or ice‑induced defects.
The Science
Primary Reaction
Controlled nucleation and crystal growth of sucrose under rapid cryogenic cooling.