Structural Characterization of Heat-Induced Protein Particles in Soy Milk
Chengang Ren, Lu Tang, Min Zhang, Shuntang Guo
Journal of Agricultural and Food Chemistry
Abstract
This study analyzed the aggregation mode of polypeptides in protein particles of soy milk by using ultracentrifugation, gel filtration, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results show that the protein particles in soy milk were mainly formed by various complex protein aggregates. These protein aggregates were mainly composed of the basic and acidic polypeptides of glycinin (11S), which interact with each other via disulfide bonds, and a very small amount of the disulfide-linked alpha' and alpha subunits of beta-conglycinin (7S). Moreover, the protein aggregates and a part of monomeric subunits of 7S and 11S form protein particles through non-covalent interactions, especially hydrophobic interactions and hydrogen bonds. It is suggested that the polymerized basic polypeptides should be located inside the protein particles, whereas the acidic polypeptides of 11S, alpha' and alpha subunits are located outside them.
Extracted Claims
3 claims extracted from this paper into the knowledge graph
protein particles in soy milk are formed by various complex protein aggregates
“The protein particles in soy milk were mainly formed by various complex protein aggregates. These protein aggregates were mainly composed of the basic and acidic polypeptides of glycinin (11S), which ...”
polymerized basic polypeptides are located inside the protein particles
“It is suggested that the polymerized basic polypeptides should be located inside the protein particles, whereas the acidic polypeptides of 11S, alpha' and alpha subunits are located outside them.”
acidic polypeptides of 11S, alpha' and alpha subunits are located outside the protein particles
“It is suggested that the polymerized basic polypeptides should be located inside the protein particles, whereas the acidic polypeptides of 11S, alpha' and alpha subunits are located outside them.”