Lactococcus lactis ssp. lactis C2 Bacteriophage sk1 Receptor involving Rhamnose and Glucose Moieties in the Cell Wall
Ruud Valyasevi, W. E. Sandine, Bruce L. Geller
Journal of Dairy Science
Abstract
Lactococcus lactis ssp. lactis C2 was reduced 64, 26, 26, 5, and 8% by addition of rhamnose, glucose, galactose, Nacetylglucosamine, and fucose, respectively, to the adsorption assay. Lectins that were specific for Nacetylglucosamine and galactose did not prevent phage adsorption. However, three lectins specific for glucose, but different in size, reduced phage adsorption from 0 to 50%. Another lectin, which was specific for rhamnose, reduced phage adsorption 80%. Rhamnose, but not glucose, partially inhibited phage infection when added to a growing culture. The data suggest that phage skl adsorbs directly to rhamnose constituents of the cell wall and that a glucose moiety is near the adsorption site or is an important conformational determinant of it.
Extracted Claims
12 claims extracted from this paper into the knowledge graph
glucose reduce phage adsorption
“Lactococcus lactis ssp. lactis C2 was reduced 64, 26, 26, 5, and 8% by addition of rhamnose, glucose, galactose, Nacetylglucosamine, and fucose, respectively, to the adsorption assay.”
rhamnose reduce phage adsorption
“Lactococcus lactis ssp. lactis C2 was reduced 64, 26, 26, 5, and 8% by addition of rhamnose, glucose, galactose, Nacetylglucosamine, and fucose, respectively, to the adsorption assay.”
glucose moiety be important conformational determinant adsorption site
“The data suggest that phage skl adsorbs directly to rhamnose constituents of the cell wall and that a glucose moiety is near the adsorption site or is an important conformational determinant of it.”